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SFB 1039

SIGNALLING BY FATTY ACID DERIVATIVES AND
SPHINGOLIPIDS IN HEALTH AND DISEASE

A05 - Macrophage polarization by saturated fatty acids under hypoxic conditions

Using cell culture models we analyze the influence of hypoxia on fatty acid-induced macrophage polarization (cytokine response, inflammasome activation, ER stress, ceramide production) and investigate the impact of b-oxidation on polarization. We further test whether hypoxia affects M2 macrophage polarization through changes in protein acetylation. Using a co-culture model, we investigate the effect of the secretome of hypoxia- and fatty acid-polarized macrophages on the insulin sensitivity of primary human adipocytes. We analyze the role of the hypoxia-induced factors (HIF) and the AMP-dependent protein kinase (AMPK) in the polarization of hypoxic macrophages in cell culture and in the in vivo model of diet-induced obesity.

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Principal Investigator

PD Dr. Dmitry Namgaladze
Institute of Biochemistry I
Faculty of Medicine
Goethe-University Frankfurt
Theodor-Stern-Kai 7
60590 Frankfurt am Main